FIG. 3.

Western blot and densitometry analysis of islet proteins in different genotypes. A: Western blotting for determining total and phosphorylated AKT. The phosphorylation of AKT at T308 and S473, and total AKT protein were examined in islets of mutant and control groups. B: Densitometry analysis for AKT phosphorylation. C: Immunoblot of p27 and FoxO1 using GAPDH as a loading control. D: Densitometry analysis for p27 and FoxO1 protein adjusted with GAPDH. E: Immunoblot for phosphorylation of S6 (top) and densitometry analysis (bottom). Figures represent blots from multiple mice in each genotype. Results are presented as relative folds of change in mutant compared with control mice. Control is Dlox. *P < 0.05 vs. all other groups or the indicated group; **P < 0.01 vs. all other groups or indicated group; §P = 0.07. Four to five mice were studied per groups. Data are presented as mean ± SEM. ADU, arbitrary density unit.