FIG. 5.

Pancreatic GLUT2 and insulin release from isolated pancreatic islets are reduced in SD rats chronically treated with MG. Either 0.9% saline or MG (60 mg/kg/day) was delivered by continuous infusion with a subcutaneous osmotic minipump for 28 days to all groups of rats (n = 6 each). The MG scavenger ALA (30 mg/kg/day in drinking water; MG + ALA) or GSH synthesis inhibitor BSO (30 mg/kg/day in drinking water; MG + BSO and BSO) was administered to some groups of rats. The control group received only saline (0.9% by pump). After 28 days, the rats were fasted overnight, and the pancreatic islets (A) were isolated as described in research design and methods. Glucose-stimulated insulin secretion was evaluated in the isolated islets in vitro. B: The pancreatic tissue was removed and processed for determination of GLUT2 protein expression by Western blotting. *P < 0.05, ***P < 0.001 vs. respective control; ††P < 0.01, †††P < 0.001 vs. respective MG group, ‡‡‡P < 0.001 vs. respective MG + BSO group.