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. 2011 Mar 1;10(1):95–110. doi: 10.1187/cbe.10-07-0098

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© 2011 S. M. DiBartolomeis. CBE—Life Sciences Education © 2011 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2: — Restriction enzyme map of a 7.3-kb wild-type D. melanogaster fragment cloned into pBluescript SK+ (bold line). This plasmid DNA is the starting material from which the 2.6-kb EcoRI (R)-digested fragment is purified and subcloned to be mapped and used as a probe to detect RFLPs between digested wild-type and dy⁷³ genomic DNAs. This exact map is available to the students in the laboratory manual. Mapping sites to the 2.6-kb fragment will complete the map for PstI (P), EcoRV (V), and BamHI (B). There are no internal HindIII (H) or XhoI (X) sites, and circled R and P sites are in the vector's polylinker. Sizes are in kilobases (kb).