Table 1.
Specific infectivity of FMDV RNA of different lymphoid tissues
| Tonsil | Spleen | Thymus | ING | RTF | MED | MES | PRE | |
|---|---|---|---|---|---|---|---|---|
| 1 | NDa (+++)b | ND (+) | ND (-) | ND (-) | ND (-) | ND (-) | ND (-) | 5 × 10-7 |
| 6 × 10-4 c | ND (+) | ND (+) | ND (-) | ND (+) | ND (-) | ND (-) | 7 × 10-8 | |
| 2 | ND (+) | 2 × 10-4 | 7 × 10-5 | 1 × 10-4 | ND (+) | ND (+) | 1 × 10-5 | 5 × 10-6 |
| 2 × 10-7 | 2 × 10-5 | 4 × 10-6 | 1 × 10-6 | ND (+) | ND (+++) | ND (+++) | 2 × 10-6 | |
| 3 | 2 × 10-4 | ND (+++) | 2 × 10-5 | ND (++) | 1 × 10-7 | 4 × 10-4 | ND (+++) | ND (+) |
| 5 × 10-4 | ND (+++) | ND (++) | ND (+) | ND (++) | ND (+++) | ND (+++) | ND (-) | |
| 3 × 10-7 | ND (+++) | ND (++) | ND (++) | ND (+) | ND (+++) | ND (+++) | ND (+) | |
| 7 | 2 × 10-7 | ND (+++) | ND (+++) | ND (+++) | ND (++) | ND (+++) | ND (+++) | ND (+) |
| ND (++) | ND (+++) | ND (+++) | ND (+++) | ND (++) | ND (+++) | ND (+++) | ND (+++) | |
| 14 | 7 × 10-6 | ND (+++) | ND (+++) | ND (+++) | ND (+++) | ND (+++) | ND (+++) | ND (+++) |
| 1 × 10-5 | ND (+++) | ND (++) | ND (++) | ND (+++) | ND (+++) | ND (+++) | ND (+++) | |
| 17 | ND (+++) | ND (+++) | ND (+++) | ND (+++) | ND (+) | ND (+++) | ND (+++) | ND (+) |
It is indicated the specific infectivity of FMDV RNA at different times post-inoculation (right columna) from tonsil, spleen, thymus and several lymph nodes [inguinal (ING), retropharyngeal (RTF), mediastinal (MED), mesenteric (MES), and prescapular (PRES)].
a ND: not determined.
b Cythopatic effect: BHK-21 cells were infected with homogenate from tissues and cytophatic effect (cpe) was evaluated at 48 h post-infection. (-), no cpe; (+), 10-20% of cpe; (++) 50% of cpe; (+++) 100% cpe.
c Specific infectivity is expressed as the number of PFU per viral RNA molecule.