Immunoreactivity according to genotype and age category and cumulative immunoreactivity according to genotype and sex for the Biosite BNP assay (A and B, respectively), the Shionogi BNP assay (C and D, respectively), and the Roche NT-proBNP assay (E and F, respectively). Please note the different scales. BNP = brain-type natriuretic peptide; IR = immunoreactivity; NT-proBNP = amino-terminal proBNP.