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. 2011 Mar 1;6(3):e14731. doi: 10.1371/journal.pone.0014731

Figure 1. NAD depletion underlies excitotoxic neuronal death.

Figure 1

(A) NAD level was measured in total cellular and mitochondrial fractions in mouse primary cortical neuron cultures exposed to NMDA 30 µM for indicated times. (mean ± SEM; n = 8; *P<0.01, difference from untreated control). (B) Cell death was assessed by measurement of LDH release (mean ± SEM; n = 8 ; *P<0.01, difference from NMDA alone). (C) NAD level was measured in neurons 4 h after exposure to NMDA 30 µM alone, NMDA plus various doses of NAD, or NAD alone (mean ± SEM; n = 4; *P<0.01, difference from untreated control; **P<0.01, difference from NMDA alone).