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. 2011 Mar 1;6(3):e14731. doi: 10.1371/journal.pone.0014731

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Fluorescence image of mitochondrial ROS detected with CMH2xROS in neuron cultures transfected with pIRES2-zsGreen1 (control vector) or pIRES2-zsGreen1-Sirt3 at 24 hrs after exposure to NMDA 30 µM. (B) The expression of control vector alone and pIRES2 ZsGreen-1-Sirt3 in transfected neurons 24 hr after exposure to NMDA 30 µM. Cell death was analyzed by counting the pyknotic bodies (shown as DAPI) in transfected neurons (mean ± SEM; n = 8; *P<0.01, difference from the culture transfected with the control vector). (C) Cell death was analyzed by LDH release in neurons transfected with Sirt3 siRNA or negative control siRNA at 24 hrs after exposure to NMDA 30 µM (mean ± SEM; n = 8; *P<0.01, difference from NMDA alone; **P<0.01, difference from NMDA plus Sirt3 siRNA).