Skip to main content
. 2011 Mar 1;9(3):e1001021. doi: 10.1371/journal.pbio.1001021

Figure 4. Knockdown of Myosin VI with siRNA blocks axonal enrichment of NgCAM.

Figure 4

In a cortical neuron transfected with siRNA against Myosin VI (MVI siRNA), surface exogenous NgCAM (A) localized both to axons and to dendrites, with an expression pattern that overlaps that of GFP (B). (C) Merge of surface NgCAM (red) and GFP (green) in a cell coexpressing MVI siRNA. In contrast, NgCAM (D) was enriched on the surface of the axon when expressed with GFP (E). (F) Merge of surface NgCAM (red) and GFP (green). When expressed with both siRNA against Myosin VI and a version of Myosin VI that is impervious to siRNA (MVIr), surface NgCAM (G) localized specifically to the axon, in contrast to the nonspecific localization of GFP (H). (I) Merge of surface NgCAM (red) and GFP (green) in a cell cotransfected with MVI siRNA and MVIr. (J) When coexpressed with either GFP alone or with MVI siRNA and MVIr, NgCAM localized to the axon with an ADR 4- to 5-fold higher than when NgCAM was coexpressed with MVI siRNA alone. Arrows point to the axon; arrowheads point to the axon initial segment. Scale bars are 10 µm. *, p<0.0001 (Wilcoxon-Mann-Whitney test).