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. 2011 Mar 1;9(3):e1001021. doi: 10.1371/journal.pbio.1001021

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Lewis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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In a cortical neuron in dissociated culture, surface NgCAMY33A (A) localizes nonspecifically when expressed with GFP-dnMVI (B). (C) Merge of NgCAMY33A (red) and GFP-dnMVI (green). In contrast, NgCAMY33A (D) localizes specifically to the axon when coexpressed with GFP (E). (F) Merge of NgCAMY33A (red) and GFP (green). (G) The ADR of NgCAM coexpressed with GFP is approximately 5-fold greater than when it is coexpressed with GFP-dnMVI. *, p<0.0001 (Wilcoxon-Mann-Whitney test). Insets show staining of endogenous Ankyrin G. Arrows point to the axon; arrowheads point to the axon initial segment. Scale bars are 10 µm.