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. 2011 Mar 1;6(3):e17296. doi: 10.1371/journal.pone.0017296

Figure 1. Salmonella infection induces PB disassembly, but not SG formation.

Figure 1

(A) HeLa cells were mock-treated or infected for 20 hours with Salmonella expressing GFP, and either left untreated (control) or treated with puromycin for 1 hour. PBs were detected by staining the cells with anti-DDX6 antibody (red channel). Scale bar, 10 µm. The region highlighted by a white square is enlarged on the rightmost panel. (B) The average number of PBs per cell in mock-treated cells and Salmonella infected cells was calculated as described in Materials and Methods. Salmonella infected cells were divided into two populations: cells in which the bacteria were not internalized (Salmonella −) and cells in which the bacteria were internalized (Salmonella +). Mean values ± standard deviations from at least three independent experiments are shown. (C) SG formation was evaluated in HeLa cells mock-treated or infected for 20 hours with Salmonella, either left at 37°C (control) or after heat-shock for 1 hour at 46°C. Cells were stained with anti-TIAR antibody (red channel).