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. 2011 Mar 1;6(3):e17581. doi: 10.1371/journal.pone.0017581

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Fischer et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Hoechst labeling of DNA in the interfollicular epidermis of wild-type and serpinB1a knockout mice. The borders of the stratum corneum and the border of the epidermis and dermis are marked with discontinuous and dotted lines, respectively. S, stratum corneum; E, epidermis; D, dermis; scale bars, 20 µm. (B) Acid DNase activity in extracts from the stratum corneum of the soles of a wild-type and a serpinB1a knockout mouse. 2 extracts were prepared from each mouse and incubated with Lambda DNA that was subsequently electrophoresed in the presence of ethidium bromide. The results are representative of investigations of 3 mice per genotype. kb, kilo-basepairs.