Figure 5. Acid DNase activity is strongly reduced in the stratum corneum of DNase 2-deficient mice.

(A) Hoechst labeling of DNA in the interfollicular epidermis of mice expressing DNase 2a (wild-type, WT) and of DNase 2a-deficient (knockout, KO) mice. The borders of the stratum corneum and the border of the epidermis and dermis are marked by discontinuous and dotted lines, respectively. S, stratum corneum; E, epidermis; D, dermis; scale bars, 20 µm. (B) Acid DNase activity in extracts from the stratum corneum of the back and the soles as well as from the surface of hair of WT and KO mice. M, molecular weight marker VI (Roche). Note that all samples shown here were derived from mice deficient in the interferon type I receptor.