Fig. 4.
LmjMCA processing during cell death.
A. L. major promastigotes were heat shocked during 10 min at 55°C then incubated during 1 h at 26°C. NP-40-soluble proteins were immunoblotted using RE53. (−): non-treated; (+): treated.
B. L. major promastigotes were treated with 1 mM hydrogen peroxide during 1 h and NP-40-soluble proteins were analysed by immunoblot with RE53. (−): non-treated; (+): treated.
C. L. major promastigotes were treated during 10 h with 40 µM miltefosine or 100 µM curcumin and NP-40-soluble proteins were analysed by immunoblot using RE53.