Skip to main content
. 2010 Dec 1;20(1):107–117. doi: 10.1002/pro.542

Figure 2.

Figure 2

Crystal structure of the p115 DH/PH domains. (a) Ribbon diagrams depicting the tertiary structure of p115 DH/PH. (b) Ribbon diagrams depicting the noncrystallographic dimer of p115 DH/PH (labeled 1 and 2). The dimer interface (marked by a box) involves a layer of β-strands near the C-terminus of one PH domain and the α4 helix from the dyad related DH domain. (c) The GEF switch in the DH domain. Electron density (cages) for the GEF switch from a 2.9 Å σA-weighted 2Fo-Fc total omit map calculated in SFCHECK (CCP4i) is contoured at 1.5 standard deviations above the mean.22 Side chains of residues 395–400 are depicted as stick models. The rest of the DH/PH domain is depicted as ribbon diagrams. (d) Trp-398 from the GEF switch is buried in a hydrophobic pocket between the GEF switch and the canonical DH domain. Side chains of residues involved are depicted as stick models. The putative hydrogen bond between side chains of Arg-399 and Glu-419 is drawn as a dotted line.