Figure 4.

Crystal and solution structures of the p115 L-DH/PH domains. (a) Ribbon diagrams depicting tertiary structures of ^ΔN2L-DH/PH (left) and L-DH/PH (right). (b) Ribbon diagrams depicting the noncrystallographic dimer of p115 L-DH/PH (labeled 1 and 2). The dimer interface (marked by a box) involves a layer of β-strands near the C-terminus of one PH domain and the α1-α2 helices from the dyad related DH domain. (c) Solution x-ray scattering profile for p115 L-DH/PH. The distance distribution function, P(r), of L-DH/PH was computed from the x-ray scattering using the program GNOM.³⁶ (d) Solution structure of L-DH/PH of p115. The solution structure (molecular envelope) is depicted as a mesh and overlapped onto the crystal structure of p115 DH/PH domains (ribbon). The crystal structure is relatively well accommodated in the molecular envelope. The large unoccupied region in the molecular envelope beneath the DH domain is likely to be the location for the linker region with limited ordered structure.