Figure 5.
Methodology for analysis of Btk-associated proteins using new TAP vector combined with mass spectrometry. A: Oriole staining of tandem affinity purified Btk complex: negative control of tandem affinity purification (TAP-Null) and tandem affinity purified Btk complex (TAP-Btk). Asterisk indicates Btk. B: Experimental workflow for TAP purification and protein identification.