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. 2010 Nov 15;20(1):140–149. doi: 10.1002/pro.546

Figure 5.

Figure 5

Methodology for analysis of Btk-associated proteins using new TAP vector combined with mass spectrometry. A: Oriole staining of tandem affinity purified Btk complex: negative control of tandem affinity purification (TAP-Null) and tandem affinity purified Btk complex (TAP-Btk). Asterisk indicates Btk. B: Experimental workflow for TAP purification and protein identification.