Figure 3.

PGE₂ induced CaP cell growth is inhibited by ERG knockdown. VCaP cells were evaluated for cell growth in the presence (+) or absence (−) of PGE₂ in response to non-targeting siRNA (NT) or ERG siRNA (E1). (A) BrdU nuclear staining of proliferating cells. In the presence of PGE₂, VCaP cells with control NT siRNA showed dramatically increased BrdU nuclear staining in response to PGE₂ treatment. In contrast, ERG siRNa transfected VCaP cells show significantly decreased BrdU nuclear staining both in the presence or absence of PGE₂. (B) Relative percent of BrdU positive cells. In control NT siRNA transfected cells, BrdU incorporation is higher in PGE₂ treated cells than in the untreated group. In contrast, BrdU incorporation is low in both ERG siRNA treated groups irrespective to PGE₂ treatment.