Figure 5.

HPGD transcript levels are lower in TMPRSS2-ERG fusion positive prostate tumor epithelial cells than in fusion negative tumor cells. Total RNa isolated from LCM prostate epithelial tumor cells and VCaP cells were used for evaluation of HPGD and GAPDH expression. Tissue expression levels of HPGD normalized for GAPDH are shown in comparison to the normalized expression levels in VCaP cells. Evaluation of HPGD expression between TMPRSS2-ERG fusion positive (+) and negative (−) prostate tumors revealed a trend towards decreased HPGD RNA expression in fusion positive tumors.