Fig. 5.

Mouse spleen signaling responses to early-stage anthrax. To tentatively discover early-stage signaling responses, the analysis described in Fig. 4 was repeated except that the ≥48 h ΔSterne and Sterne data were excluded. Twenty-six phosphopeptides were tentatively found to be altered (q<0.5). Note that by requiring q<0.5, 50% of the data (13 phosphopeptides) were estimated to have been wrongly considered significant by the ANOVAs. Of these 26 phosphopeptides, ten were derived from eight proteins that have known roles in the immune system. Two of these eight proteins, Pram1 and Hemgn, each had two phosphopeptides that responded similarly (i.e. clustered together) at 24 h postchallenge, indicating that both proteins were probably genuinely affected during early-stage anthrax.