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. 2010 Nov 19;10(3):M110.001552. doi: 10.1074/mcp.M110.001552

Table I. Bioinformatic Src and p38 MAPK phosphorylation motif predictions. Identified phosphopeptides were submitted to GPS 2.1 to predict potential p38 MAPK and Src Tyr kinase motifs. Proteins were grouped by molecular function and the NCBI accession number, the phosphopeptide identified in the proteomic analysis, and the phosphorylated residue(s) listed. A raised numeral indicates the amino acid-specific phosphorylation of serine, threonine, or tyrosine. Phosphorylated amino acid(s) predicted to comprise a Src family phosphorylation motif are denoted by Y. Those comprising a potential p38 MAPK phosphorylation motif are denoted by S and T.

Identified Protein Accession Number Peptide sequence Phosphorylated Residue
Adhesion
Plakophilin 2 isoform 2b 4758932 LEIS151PDS154SPER S151, S154
Cell cycle
    Retinoblastoma-like protein 1 isoform b 34577077 EKEAVIT385PVASATQSVS395R T385
Cytoskeletal regulation
    Drebrin 1 isoform b 18426913 S339PS341DSST345AS347T348PVAEQIER S339, T348
GTPases and GTPase-binding proteins
    ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 19923540 S862PEDGPSAR S862
    Exophilin 5 (Slac2-b) 21359818 TSCDSLDLSSAALPDS887SPS890K S887
    Ras protein-specific guanine nucleotide-releasing factor 1 isoform 24797099 S232PHADSMR S232
Immune/inflammatory response
    Lactotransferrin; transferrin 4505043 PVAAEVY102GT104ER Y102
Ion/Metabolite Transport
    Barttin 17017967 S91PSPQPPY98VR S91, Y98
Kinase/Phosphatase
    G protein coupled receptor kinase 5 55958649 GESMALNEKQILEKVNSQFVVNLAY251A-Y253ETK Y251
    Serine/Threonine kinase SMG-1 isoform 2 14719394 SSCT2305T2306PDEWW T2306
    PREDICTED: similar to protein phosphatase 2A 48 kDa regulatory 51472904 KT75GPRACSTPLSTSS88PS90PR S88, S90
Miscellaneous
    Arsenate resistance protein 2 33383231 DEHSS89DPY92 Y92
    T-complex protein 1 subunit theta 42662316 IGLS124VSEVIEGY132EIACRK Y132
Receptor/signal transduction
    Aryl hydrocarbon receptor 4502003 SS90PTERNGGQDNC S90
    Chemokine (C-X-C motif) receptor 4 isoform b 4503175 T13EEMGSGDY21 Y21
    G-protein coupled receptor 88 11545753 DGAGGSY112 Y112
    Olfactory receptor, family 6, subfamily C, member 76 41201775 PLHY129TTIMS134DR Y129
Redox
    Cytochrome b5 reductase b5R.2 isoform 1 47778923 AYT70PVSSDDDR T70
Secreted
    Angiogenin, RNase A family, 5 precursor 4557313 GLTS61PCK S61
Structural proteins
    Fibronectin 1 isoform 3 preproprotein 16933542 GDS1527PASS1531K S1527
    Usherin isoform B 46249406 S442NFTPY447SRGNVT453F Y447
Translation/transcription
    Protein CASP 31652240 Y493SKAMQEAGSTSMIFSTGPY Y493
Ubiquitination
    E3 SUMO-protein ligase RanBP2 638207 VT2027GEEDEKVLY2036 Y2036
Unknown Function
    AlkB, alkylation repair homolog 5 40353210 Y60QEDSDPER Y60
    Hypothetical protein LOC220108 31542760 SSS459DLS462VVS465AY467 Y467
    Hypothetical protein LOC348487 32699058 CFQTTNGY67LSDS71R Y67
    Nucleoporin 214kDa 33946327 HGAPS1081PS1083HPISAPQAAAAAALR S1081
    PREDICTED: NHS-like 1 isoform 1 51465299 SRTPPSAASSRY1511SMR Y1511
    Testis expressed sequence 15 13775174 LINVCGDS1842PK S1842
    Tetratricopeptide repeat domain 37 7662078 IKLSDY366DSS369EEAIRT375L Y366