Fig. 1.

Altered cell morphology and increased cell motility of PRA1-knockdown cell clones. A, PRA1-knockdown clones (K3–2, K3–14) exhibited elongated and fibroblast-like shape when grown at subconfluence, whereas control clones (C15, C15–3) showed epithelial morphology with relatively strong cell-cell contacts. Scale bar, 100 μm. B, A wound healing assay was performed to track cell migration of two PRA1-knockdown clones and two control clones. Cells were seeded in 60-mm plates at high density and allowed to form monolayers overnight. After wounding with a pipette tip, the wound healing activity was observable between 3 and 24 h. By 16 h, cell migration could be easily differentiated between cells with a low magnification (10×) objective. Scale bar, 100 μm.