Temporal and spatial patterns of BBB opening in the hippocampus following TFI. (A, B) Western blot analysis of IgG showing increase in the light and heavy chains of IgG after sham operation (Ctrl) or TFI. Levels of IgG were measured at indicated points of time after TFI, normalized to those of β-actin, and compared to control (n = 4-5 rats per group). (C) Fluorescent photomicrographs showing EBD-albumin extravasation and cerebral endothelium stained with EBA, in the hippocampal formation, 48 h after Ctrl or TFI. Note the dramatic increase in EBD extravasation in the endothelium (yellow), primarily in the alveus (Alv), stratum oriens (SO), and stratum pyramidale (SP) of the CA1-2 areas. Abbreviations: DG, dentate gyrus; H, hilus, SR, stratum radiatum. Scale bar: 1.0 mm.