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. 2011 Jan 31;43(2):121–128. doi: 10.3858/emm.2011.43.2.020

Figure 3.

Figure 3

Iron overload and free radical production in the CECs after TFI. (A) Brightfield photomicrographs of the hippocampal CA1 area after Perls iron staining following sham operation (Ctrl) or 0.5, 2, and 8 h following TFI. Scale bar, 50 µm. (B) Brightfield and fluorescence photomicrographs of adjacent hippocampal CA1 sections after Perls staining (top panel) or immunolabeling with EBA (bottom panel) 0.5 h after TFI. Arrowheads indicate iron overload in endothelial cells. Scale bar, 50 µm. (C) Superimposed images of fluorescence photomicrographs of hippocampal sections labeled with EBA and MitoTracker Red CM-H2XROs (MT, red), a mitochondrial free radical probe, 2 h after Ctrl or TFI, alone or with administration of DFO (200 mg/kg, s.c.) or Neu2000 (50 mg/kg; i.p.) immediately after reperfusion. Abbreviations: Endo, endothelium; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum. Scale bar, 50 µm.