DFO and Neu2000 protect cerebral endothelial cells against TFI. (A) Western blot analysis of IgG and TJ proteins 48 h after Ctrl or TFI, alone or with administration of DFO (200 mg/kg, s.c.) or Neu2000 (50 mg/kg; i.p.) immediately after reperfusion (n = 3-4 rats per group). *P < 0.05 compared to Ctrl; #P < 0.05 compared to TFI. (B) Fluorescent and brightfield photomicrographs of hippocampal CA1 sections labeled with EBD-albumin (red, top panel) or TUNEL and methyl green, a chromatin staining dye (bottom panel) 48 h after Ctrl or TFI, alone or with administration of DFO (200 mg/kg, s.c.) or Neu2000 (50 mg/kg; i.p.) immediately after reperfusion. Arrowheads marked the endothelium and CECs of CA1-2 areas. Abbreviations: Alv, alveus; Endo, endothelium; SO, stratum oriens; SP, stratum pyramidale. Scale bar, 50 µm.