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. 2010 Dec 28;43(2):63–70. doi: 10.3858/emm.2011.43.2.008

Figure 1.

Figure 1

Construction of the CCL22 miRNA expression vector (miRNACCL22) and miRNA expression of recombinant S. typhimurium expressing CCL22 miRNA. The dsDNA oligo of CCL22 cloned into the pcDNATM6.2-GW/EmGFP-miR expression vector using T4 DNA ligase (A). To examine the expression of the miRNA vector and S. typhimurium-expressing CCL22 miRNA, Raw 264.7 cells were infected with ST-miRCCL22, ST-miRCV or EmGFP negative controltransfected counterparts. Expression of the miRNA vector was observed in cells with ST-miRCCL22 and ST-miRCV (B). GFP from recombinant Salmonella was also detected in ST-miRCCL22 and ST-miRCV by Western blots, but not in control samples (C).