Figure 4.
AGR2 overexpression increased cell growth and cell migration of human ovarian cancer cells. (A) Control vector- and AGR2-overexpressing stable 2774 cells were plated onto 96-well sensor plates at 2.5 × 103 cells per well, then incubated overnight. Growth of cells was continuously monitored using real-time cell electronic sensing (RT-CES) system. (B) Enhancement of cancer cell migration by AGR2 overexpression. Cancer cell migration was assayed using Transwells after coating the lower surface of the filter with gelatin. The number of cancer cells that migrated to the lower side of the filter was counted. The results are the means ± SD (*, P < 0.05 compared to 2774-CON).