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. 2010 Dec 15;96(3):E546–E553. doi: 10.1210/jc.2010-1594

Fig. 5.

Fig. 5.

Direct interaction of miRs and THRB (luciferase assay) and impact on TRE (promoter assay). A, Dual luciferase assay. The relative luciferase activity of reporter constructs with the 3′-UTR of THRB in the presence of pre-miRs or scrambled pre-miR (negative control). Data are expressed as mean values ± sem. B, Promoter assay. The relative luciferase activity of reporter constructs with two positive TRE sequences cloned in the promoter region of luciferase gene in the presence of pre-miRs or scrambled pre-miR (negative control). Data are expressed as mean values ± sem. ***, P < 0.0001; **, P < 0.01.