Figure 5.

Oscillatory changes in cytosolic Ca²⁺ in response to application of carbachol (CCh). A. Representative traces of Ca²⁺ dynamics induced by CCh in the absence of extracellular Ca²⁺. 20 μM CPA was applied following CCh evoked response to assess ER Ca²⁺ content. CPA-evoked Ca²⁺ release following treatment with CCh shows a concentration dependent decrease in ER store content. B. A representative trace showing determination of rate of influx by exponential fit (red colored trace) to the slope of Mn²⁺-induced fura-2 fluorescence quench. C. Representative traces of unstimulated (-CCh) influx and following application of 30 μM CPA or 100 μM CCh in the presence or absence of 1μM Gd³⁺ or BTP2 to measure influx (chevron) determined by Mn²⁺ quench method in fura-2 loaded BON cells. D. Averaged rates (t) of influx under different conditions of activation and block of SOCE.