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. 2009 Dec 2;2(1):29–35.

Figure 1.

Figure 1

Immunoblotting analysis of proteins from control and AD patient brain tissues. A: Immunoblotting with anti-rabbit secondary antibody alone detected high cross-immunoreactivity in aged human cortices of both the control group (lane 1∼5) and the AD group (lane 6∼11). B: Immunoblotting with anti-mouse secondary antibody alone showed no cross-immunoreactivity in all cases. C: Immunoblotting with anti-goat secondary antibody alone detected weak cross-immunoreacticity in both the control group (lane 1∼7) and the AD group (lane 8∼14). D: Treatment with a blocking buffer containing both 5% milk and 5% goat serum does not prevent nonspecific immunoreactivity with anti-rabbit secondary antibody (same lane sequence as panel C).