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. 2011 Feb 25;2(3):696–704. doi: 10.1364/BOE.2.000696

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 1. — (a) Microscopy Setup. HWP: half-wave plate, PC: polarizer cube, S: camera-controlled shutter, T: telescope comprised of two achromatic lenses (f = −75 mm and +125 mm), SLM: spatial light modulator, FP: Fourier plane, F_F: Fourier achromatic lens (f = 300 mm), DG: diffractive grating, IP: image plane, F_G: achromatic lens for collimating the diffracted beam (f = 300 mm), Hg: mercury arc lamp, M₁: Flip mirror, DM: excitation-detection dichroic filters, O: microscope objective, CCD: EMCCD camera. (b) Fluorescence intensity of a thin layer of fluorescein scanned through the focal plane with and without TF by replacing the DG by a mirror. (c) Fluorescence of live cultured cells loaded with Fluo-4 AM calcium indicator. (Green) Wide-field mercury arc lamp excitation (λ_exc = 450 nm, λ_em = 525 nm longpass), (Dashed line) targeted illumination, (Red) TF patterned illumination (λ_exc = 800 nm, λ_em = 535/40 nm). All scale bars are 20 µm.