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. 2011 Mar 2;6(3):e17151. doi: 10.1371/journal.pone.0017151

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Zeitlin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Quantitative real-time RT-PCR was used to detect levels of UNG transcript remaining after siRNA transfection in U20S cells. A. Western blot analysis of endogenous UNG2 and CENP-A protein levels 48 hours after siRNA transfection in U20S cells. Tubulin was detected as a loading control. A. Normalized band intensity for part A. B. Quantitative real-time RT-PCR was used to detect levels of UNG transcript remaining after siRNA transfection in HeLa cells. B. Western blot analysis of endogenous UNG2 and CENP-A protein levels 24 hours after siRNA transfection in HeLa cells. Histone H3 was detected as a loading control. B. Normalized band intensity ratios for part B. C. Normalized mitotic index of HeLa cells 24 hours after transfection with control or UNG-directed siRNA ( cells per sample). (unpaired t-test).

Inline graphic — A. Quantitative real-time RT-PCR was used to detect levels of UNG transcript remaining after siRNA transfection in U20S cells. A. Western blot analysis of endogenous UNG2 and CENP-A protein levels 48 hours after siRNA transfection in U20S cells. Tubulin was detected as a loading control. A. Normalized band intensity for part A. B. Quantitative real-time RT-PCR was used to detect levels of UNG transcript remaining after siRNA transfection in HeLa cells. B. Western blot analysis of endogenous UNG2 and CENP-A protein levels 24 hours after siRNA transfection in HeLa cells. Histone H3 was detected as a loading control. B. Normalized band intensity ratios for part B. C. Normalized mitotic index of HeLa cells 24 hours after transfection with control or UNG-directed siRNA ( cells per sample). (unpaired t-test).