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. 2011 Mar 2;6(3):e17151. doi: 10.1371/journal.pone.0017151

Figure 7. Cell cycle staging of UNG2 localization during G2 using indirect immunofluorescence.

Figure 7

Example images from high-resolution confocal imaging using four channels. Left column, DNA was detected with DAPI (blue), endogenous CENP-A with ACA (red), and endogenous UNG2 with PU59 (green). Second column from the left, Aurora B was detected with Cy5 (pink) shown here with DNA (blue). Aurora B and UNG2 channels are shown alone in grayscale for clarity. Note the presence of Aurora B in the second row, indicating that UNG2 foci resembling centromeres are prominent during early G2.