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. 2011 Mar 2;6(3):e17352. doi: 10.1371/journal.pone.0017352

Figure 2. DAP independent growth is restored by expression of dapA in single or multi copy.

Figure 2

Wild type KIM D27, and isogenic strains KIM D27-1003, KIM D27-1003pdapA, or KIM D27-1011 were grown overnight in HIB at 26°C, then diluted to an OD600 of .075 and grown for 25 hrs at 26°C (A) or 37°C (B) with shaking at 130 rpm, monitoring OD600 as indicated. KIM D27-1003 strain with no DAP supplementation was washed 3X in sterile PBS to remove excess DAP from the overnight culture. Data are representative of 3 independent experiments.