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. 2011 Mar 2;6(3):e17590. doi: 10.1371/journal.pone.0017590

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Jászai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) Cryosections of chick embryos (E5) were processed for non-radioactive ISH using either antisense (A, C, D; Prom1) or sense (B; Prom-1S) DIG-labelled prominin-1 probe. The boxed areas in panel A are shown at higher magnification in panels C and D. Dashed lines delimit the central retinal primordium and white arrow indicates prominin-1 expression. Black arrowhead and arrow point to the peripheral retinal primordium and corneal epithelium, respectively, being weakly positive for prominin-1. White arrowheads indicate the prominin-1–positive anterior lens (L) epithelium. Scale bars, A and B, 250 µm; C and D, 50 µm.