Figure 11. The tabular summary provides values for the three parameters that define a cycle.

C: cycle number, F_C: the fluorescence reading, E_C: the cycle's amplification efficiency, %Av. Fo: the percent difference between the cycle F₀ and the average F₀ generated by the cycles within the LRE window (designated by the red font). In this example, the F₀ threshold (Figure 9) was set to 6% so that cycle 29, which generated a 6.84% difference, triggered termination of LRE window expansion at cycle 28.