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. 2011 Mar 2;6(3):e17636. doi: 10.1371/journal.pone.0017636

Figure 14. Excel summary of the cDNA quantifications contained within the demonstration experiment database.

Figure 14

Expressed as the number of transcripts per 2.5 ng of total RNA, these datasets provide insights into both the utility of absolute quantification and the precision that can be generated by the LRE Analyzer. This reveals a run-to-run CV of ±5–16% for all three targets, and a RT-to-RT CV of ±1–20%. This summary also presents LDA quantifications that provide an independent determination of target quantity, with the difference expressed as a percentage (%Diff). Note that the GAPDH LDA failed due to the frequent production of non-specific products when the target quantity was diluted below one molecule per aliquot.