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. 2011 Mar 2;6(3):e17637. doi: 10.1371/journal.pone.0017637

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Rigamonti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. The rate of adipose tissue replication was assayed by co-staining for the cell cycle protein Ki67 (green) and the preadipocyte/adipocyte marker C/EBPα (red) in 14-week-old wildtype and OB/OB mice. Representative images of wildtype and OB/OB adipose tissue are shown. White arrows indicate cells co-staining for Ki67 and C/EBPα. DAPI stained nuclei are blue. Magnification, 200x; scale bars 100 µm. B. The percentage of C/EBPα-expressing cells in the growth cycle was quantitated for 14-week old C57/BL6 wildtype (n = 9) and OB/OB (n = 6) mice. There is a statistically significant increase in the rate of preadipocyte replication (p<0.05) from 4.6% to 7.3%, in wildtype and OB/OB mice, respectively. C. The rate of adipose tissue replication was assayed by co-staining for BrdU incorporation (green) and the preadipocyte/adipocyte marker C/EBPα (red) in 14-week-old wildtype and OB/OB mice. Representative images of wildtype and OB/OB adipose tissue are shown. White arrows indicate cells co-staining for BrdU and C/EBPα. DAPI stained nuclei are blue. Magnification, 200x; scale bars 100 µm. D. The percentage of C/EBPα-expressing cells having incorporated BrdU following 5 days of BrdU injection was quantitated for 14-week old C57/BL6 wildtype (n = 5) and OB/OB (n = 6) mice. There is a statistically significant increase in the rate of adipose tissue replication (p<0.05) from 2.9% to 9.7%, in wildtype and OB/OB mice, respectively.