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. 2011 Mar;137(3):299–314. doi: 10.1085/jgp.201010557

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© 2011 Pratt et al.

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Figure 7. — Decrease of ATP sensitivity in response to PIP₂ is abrogated by the E128K mutation. (A) Representative inside-out patch voltage clamp recordings from COSm6 cells transfected with WT (top) or E128K (bottom) K_ATP channels. Bars: WT, 1 min and 1,000 pA; E128K, 1 min and 500 pA. Zero current is indicated by the dotted lines. (B and C) Current inhibition by ATP was assessed using several ATP concentrations (0.01, 0.1, and 1 mM) before PIP₂ exposure (filled black symbols) and after three 30-s exposures to 5 µM ATP (open gray symbols) for WT (B) and E128K (C) channels. A best fit for each dose–response was calculated using the Hill equation (before PIP₂ exposure: solid lines; after PIP₂ exposure: dotted lines). Error bars represent SEM. n = 4–8 patches for each data point.