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. 2011 Mar;137(3):315–325. doi: 10.1085/jgp.201010582

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© 2011 Gustina and Trudeau

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Figure 5. — Biochemical interaction of hERG 1–135 with the CNBD. (A) Schematic of the hERG channel and the constructs used for the biochemical interaction assay. (B) Coomassie stain (CB, Coomassie blue) and Western blot of GST N1–135 and GST-only control probed with α-Flag to detect bound hERG H₆ C-linker/CNBD-Flag.