Table 1.

Metabolomic analysis of mammary fat pads from Cav-1 (−/−) deficient mice

Amino Acids:

Alanine and aspartate metabolism: alanine (1.7); asparagine (2.3); aspartate (2.7)

Cysteine, methionine, SAM, taurine metabolism: cysteine (1.6); hypotaurine (1.8); methionine (2.2); N-acetylmethionine (2.7); S-adenosylhomocysteine (SAH) (0.7)

Glutamate metabolism: glutamate (1.9); glutamine (1.6); N-acetyl-aspartyl-glutamate (NAAG) (1.6)

Glutathione metabolism: 5-oxoproline (1.5); cysteine-glutathione disulfide (1.4); glutathione, oxidized (GSSG) (1.5); glutathione, reduced (GSH) (1.7)

Glycine, serine and threonine metabolism: glycine (2.3); serine (2.5); threonine (2.0)

Histidine metabolism:histamine (2.5); histidine (2.4); urocanate (3.0)

Lysine metabolism: lysine (1.7); pipecolate (1.9)

Phenylalanine & tyrosine metabolism: phenylalanine (2.3); tyrosine (2.5)

Tryptophan metabolism:5-hydroxyindoleacetate (2.7); C-glycosyl-tryptophan (1.7); tryptophan (2.3)

Urea cycle; arginine-, proline-, metabolism: arginine (1.9); assymetric dimethylarginine (ADMA) (3.3); proline (2.3); trans-4-hydroxyproline (2.0); urea (2.5)

Valine, leucine and isoleucine metabolism:isoleucine (2.5); leucine (2.3); valine (2.0)

Peptides:

Dipeptide:glycylproline (2.8); proline-hydroxy-proline (1.8)

Gamma-glutamyl: gamma-glutamylglutamate (1.7); gamma-glutamylisoleucine (2.0); gamma-glutamylleucine (1.9); gamma-glutamylphenylalanine (1.8); gamma-glutamyltryptophan (2.1); gamma-glutamyltyrosine (1.9); gamma-glutamylvaline (1.7)

Carbohydrates:

Aminosugars metabolism: N-acetylglucosamine 6-phosphate (1.6)

Fructose, mannose, galactose, starch and sucrose metabolism: erythrose (2.0); fructose (2.0); mannose-6-phosphate (1.8)

Glycolysis, gluconeogenesis, pyruvate metabolism: fructose-6-phosphate (1.9); glucose (1.5); glucose-6-phosphate (G6p) (1.9); pyruvate (1.4)*

Nucleotide sugars, pentose metabolism: ribose (1.9); sedoheptulose-7-phosphate (1.7)

Sugar alcohol: myo-inositol (1.4)

Cofactors and vitamins:

Ascorbate and aldarate metabolism:ascorbate (Vitamin C) (11.2); threonate (1.4)

Nicotinate and nicotinamide metabolism: nicotinamide (1.3)

Pantothenate and CoA metabolism: pantothenate (1.4)

Riboflavin metabolism:riboflavin (Vitamin B2) (2.6)

Tocopherol metabolism:alpha-tocopherol (2.7)

Vitamin B6 metabolism: pyridoxate (1.8)

Energy:

Krebs cycle: fumarate (1.6); malate (1.4)

Oxidative phosphorylation: methylphosphate (1.6); phosphate (1.3)

Lipids:

Carnitine metabolism: 3-dehydrocarnitine (1.3); carnitine (0.9); malonylcarnitine (0.6)

Essential fatty acid: dihomo-linolenate (20:3n3 or n6) (1.5)

Fatty acid, ester: n-Butyl oleate (1.5)

Glycerolipid metabolism: choline (1.3); ethanolamine (2.1) glycerol (1.2); phosphoethanolamine (2.4)

Inositol metabolism: inositol 1-phosphate (I1p) (2.4)

Ketone bodies:3-hydroxybutyrate (BHBA) (4.3)

Long chain fatty acids: arachidonate (20:4n6) (1.5); margarate (17:0) (1.4); myristoleate (14:1n5) (1.8)

Monoacylglycerol: 1-stearoylglycerol (1-monostearin) (1.4)

Sterol/Steroid: cholesterol (1.6)

Nucleotides:

Purine metabolism, (hypo)xanthine/inosine containing: hypoxanthine (1.6); inosine (0.7); xanthine (1.7); xanthosine (1.3)

Purine metabolism, adenine containing: adenosine 2′-monophosphate (2′-AMp) (2.4); N1-methyladenosine (2.0)

Purine metabolism, guanine containing: guanosine (0.4)

Purine metabolism, urate metabolism: urate (0.7)

Pyrimidine metabolism, cytidine containing: 2′-deoxycytidine (2.2); cytidine (2.5); cytidine 5′-monophosphate (5′-CMp) (0.8); cytidine-3′-monophosphate (3′-CMp) (1.9)

Pyrimidine metabolism, uracil containing: pseudouridine (1.7); uracil (3.8)

Xenobiotics:

Benzoate metabolism: 4-ethylphenylsulfate (2.1); catechol sulfate (2.5); hippurate (1.8)

Fold-changes (KO/Wt) are shown in parentheses for all metabolites that showed a significant change (p ≤ 0.05). In one case, an asterisk (*) indicates p ≤ 0.1. Metabolites showing an increase of 2.5 or greater are underlined. All other p values were p≤0.05.