Figure 4.

Oxidative stress-induced autophagy correlates with Cav-1 downregulation. BSO treatment downregulates Cav-1 while promoting autophagy. To induce oxidative stress, hTERT-fibroblasts were treated with increasing concentration of BSO (1 µM and 1 mM) for 48 hours. Cell lysates were then analyzed by Western blot analysis using anti-Cav-1 and LC3B antibodies. Note that Cav-1 levels are greatly decreased upon treatment with 1 mM of the pro-oxidant BSO. At the same concentration of BSO, the accumulation of the active LC3B-II form is highest. β-actin was used as a control to assess equal protein loading.