Figure 14.

Activated HIF1a in Human Breast Cancer Cells Does Not Appear to Induce Mitophagy. (A) Mitochondrial respiratory complexes. To assess the status of the mitochondrial respiratory chain, breast cancer cell lysates were prepared and subjected to immunoblot analysis with a battery of antibodies directed against mitochondrial complex components (I–V). Note that expression of activated HIF1a does not affect components of mitochondrial complexes I–V. Immunoblotting with beta-actin is shown as a control for equal loading. (B) BNIP3L expression. Note that expression of HIF1a (wild-type or mutationally-activated) does not affect BNIP3L expression. Immunoblotting with beta-actin is shown as a control for equal loading.