Figure 5.

Localization of mitophagy markers (BNIP3 and BNIP3L) in activated HIF1a transfected fibroblasts. BNIP3 and BNIP3L are two distinct genes which are markers of mitophagy, and are thought to be under the transcriptional control of HIF1a activation. Note that both BNIP3 (A) and BNIP3L (B) are upregulated in fibroblasts harboring activated HIF1a, as compared with the vector alone control (pBABE). Both show a morphological pattern that is consistent with mitochondrial and/or autophagosomal localization, as expected.