Table 1.
Relative mRNA quantitations
| Gene | RPKM HeLa | RPKM H9 | Function |
| Gapdh | 1230 | 1727 | Internal control |
| Nanog | 0.05 | 21.8 | Stem cell marker |
| Sox2 | 0.00 | 70.6 | Stem cell marker |
| Lin28 | 0.03 | 285.0 | Stem cell marker |
| Oct3/4 | 0.2 | 560 | Stem cell marker |
| TLR3 | 1.3 | 0.1 | IFNβ induction |
| TICAM-1/TRIF | 11.8 | 0.4 | IFNβ induction |
| RIG-I | 0.8 | 1.0 | IFNβ induction |
| STING/MITA | 18.1 | 0.1 | IFNβ induction |
| TBK1 | 6.0 | 2.0 | IFNβ induction |
| IKBKE | 2.0 | 3.3 | IFNβ induction |
| MDA5 | 1.9 | 0.2 | IFNβ induction |
| VISA/IPS-1/MAVS | 6.9 | 6.8 | IFNβ induction |
| EYA4 | 48.6 | 0.0 | IFNβ induction |
| PKR | 14.1 | 4.1 | IFNβ induction |
| NFκB1 | 8.3 | 1.8 | IFNβ induction |
| NFκB2 | 16.2 | 6.3 | IFNβ induction |
| IκB | 6.3 | 4.6 | IFNβ induction |
| IRF3 | 10.9 | 19.3 | IFNβ induction |
| IRF7 | 0.4 | 2.6 | IFNβ induction |
| OAS1 | 8.7 | 0.05 | OAS, RNAseL pathway |
| OAS3 | 12.7 | 0.4 | OAS, RNAseL pathway |
| RNAse L | 0.68 | 0.1 | OAS, RNAseL pathway |
| ADAR1 | 58.3 | 53.5 | Editing (nuclear) |
Relative expression of genes involved in the cytoplasmic pathways to dsRNA between HeLa and H9 cells. Equal amounts of poly(A)+ RNAs were analyzed using the Illumina Genome Analyzer. The relative levels of mRNA expression are reported as the normalized number of reads that mapped to the annotated exons of each gene (# of reads per thousand nucleotides of exons, per million total reads [RPKM]). Higher numbers are positively correlated to higher levels of gene expression. Reads from one experiment are reported, but similar relative levels of mRNA expression were seen in two other independent sequencing experiments.