Fig. 1.

Confirmation of lumican overexpression and efficient secretion (a) Confirmation of secreted lumican transgene expression was performed on conditioned media from the MCA102 fibrosarcoma and Pan02 pancreatic adenocarcinoma cell lines. TCA/DOC precipitation and detection with polyclonal anti-lumican antibodies revealed little or no endogenous lumican expression. Ni-Ag precipitation and detection with anti-cMyc antibody confirmed overexpression of Myc-tagged lumican. (b) Detection of intracellular lumican was performed on whole cell lysates from the MCA102 and Pan02 cell lines using polyclonal anti-lumican antibodies. Overexpressed lumican was detected using anti-cMyc antibody. Stripping and reblotting with β-actin antibodies served as a loading control