Fig. 2.

Lumican overexpression exhibits pleiotropic effects in vitro (a) Lumican-overexpressing cell lines and their corresponding control cell lines were induced to invade through Matrigel coated Boyden chambers. Lumican overexpression increased invasion in PanO2 cells but decreased invasion in MCA102 cells. Data is the average (+/-SEM) of at least four independent experiments. (b) The anchorage independent growth of lumican-overexpressing and control cells was examined by culturing cells in soft-agar. The volumes of the resulting colonies were calculated from two dimensional measurements of colony diameter. Lumican increased MCA102 colony size but decreased PanO2 colony size. Data is presented as the average of three independent experiments (+/-SEM). (c) Lumican-overexpressing PanO2 and MCA102 cells were cultured for 1–4 days. Each day, the relative number of cells was measured with WST-1 cell proliferation reagent. Lumican increased PanO2 proliferation, but did not affect MCA102 proliferation. Data is presented as the average of four independent experiments (+/-SEM). (* indicates p < .05, Student’s T-Test)