Fig. 3.

Real-time (RT)-PCR for angiopoietin (a), IGFBP-3 (b), ferredoxin 1 (c), BMP (d), decorin (e), and fibulin 1 (f) was performed on total RNA extracted from tumour biopsies deriving from the malignant tissue of five patients with osteoblastic osteosarcoma and from five patients with non-osteoblastic osteosarcoma. Differences in gene expression levels between the two groups were analysed by Student’s t test. Primers and cycling conditions for each of the amplified genes are described in Materials and Methods