Figure 5.

Invasion phenotypes of three laboratory strains of P. falciparum. Unlabeled pRBC were incubated with CFDA-SE- or DDAO-SE-labeled RBC, at an unlabeled-to-labeled ratio of 1:2 and a starting parasitemia of 1%. After 48 h, parasites were stained with Hoechst 33342 or SYBR Green I, respectively, and final parasitemia in the target population was determined by gating on the fluorescently labeled RBC population using flow cytometry. Invasion efficiencies were determined as a percentage of the final parasitemia of a mock-treated-labeled positive control RBC group.