Figure 2.

Menstrual levels of IL-8 can up-regulate network formation in HUVECs. A: The number of capillary tube branch points formed by HUVECs significantly increased when treated for 8 hours with conditioned medium (CM) from menstrual endometrial biopsy specimens cultured for 24 hours in vitro versus control medium. No such increase was seen with conditioned medium from mid secretory explants (n = 4 or 5). B: The number of capillary tube branch points formed by HUVECs treated with 20 ng of human recombinant IL-8 (M, menstrual levels) was significantly greater that those formed when treated with control medium or 0.5 ng of IL-8 (MS, mid secretory levels) (n = 3). *P < 0.05.