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IL-8 mRNA up-regulation by PGE2 and hypoxia depends on cycle stage. A: IL-8 mRNA expression in early-mid-secretory endometrium (n = 7) after in vitro culture for 24 hours under normoxic conditions with vehicle (NV), normoxic conditions (21% O2) with 100 nmol/L PGE2 (NPGE2), or hypoxic conditions (0.5% O2) plus vehicle (HV). B: IL-8 mRNA expression in proliferative endometrium cultured in identical conditions (n = 3). *P < 0.05. ns, not significant.
